FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679363
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT30170.44409248075034996No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.4315807602121399No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.31323460359189415No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.21122728202742866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8250.053.5807232
GTGGTAT8350.053.4598731
GTATCAA35450.045.331561
TATCAAC39350.040.9377172
GGTATCA18100.039.459881
TCAACGC42950.037.599814
ATCAACG43400.037.3078733
CAACGCA43850.036.8280945
AACGCAG45200.035.8221636
CGCAGAG48650.033.366748
ACGCAGA49500.032.8796277
GCAGAGT51250.031.7569059
CAGAGTA34500.027.22114810-11
GCATGCG808.6142524E-426.5590959
CGTCAGA1159.171092E-625.8681555
GAGTACG37100.025.08438512-13
CGGGATA1650.024.46656216-17
AGAGTAC33200.023.67919510-11
GTACGGG40500.022.71622514-15
TATCTCG950.002342871222.3671995
Produced by FastQC (version 0.11.5)