FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740211
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23460.13481123840729659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC34500.038.6409382
GTGGTAT34900.038.2878231
GTATCAA113900.025.4911841
TATCAAC125300.023.0775472
GGTATCA60500.022.7585663
AGAGTAC82450.022.55047611
CAGAGTA84100.022.3036310
ATCAACG136350.021.0631713
TCAACGC136800.020.9537054
AACGCAG138100.020.7763586
CAACGCA139600.020.5519355
GTCCGGA1754.083631E-918.8573455
ACGCAGA155550.018.4825427
CCTCGCG750.007414159418.3329989
GCCCGAC750.00742277118.3293130
CGCAGAG157300.018.229748
AGTACGG101600.018.13763813
GTTATAC2001.1114025E-917.8638863
GAGTACG103700.017.82338312
GCAGAGT163150.017.5969819
Produced by FastQC (version 0.11.5)