FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914329
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.43999479399647173No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.28075233313172826No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT22060.2412698273816099No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.18669428619238806No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC13350.1460087124000223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11750.044.140751
TGGTATC11650.043.0458532
GTATCAA36700.038.9216271
GGTATCA19900.038.4536171
TATCAAC40750.035.041842
TCAACGC43350.032.8421024
ATCAACG43450.032.7665183
CAACGCA44500.031.9933765
AACGCAG45100.031.5677436
ACGCAGA48450.029.5588557
CGCAGAG50500.028.3589428
GCAGAGT53750.026.5651539
CAGAGTA31550.025.86147910-11
GAGTACG37600.021.64374712-13
GTATAAC1656.3553525E-620.6121751
AGAGTAC30150.020.29675710-11
GTACGGG42500.018.94836614-15
TACGGGT4700.018.0845364
GTATGAC2153.1259515E-617.7901363
GCTTGAC2656.6063876E-817.6467461
Produced by FastQC (version 0.11.5)