FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length91
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33.614457831325301No Hit
GTTTAAATGACTGTTATTCAACTTTCACATCAAAAACTAATGACAACGAA11.2048192771084338No Hit
GTGTTTGCCCCATAAATAGCCTTTGGACTGCATTATTTTGACAAAATACT11.2048192771084338No Hit
GAAGAAGAAGATCAGCACTGTGGTGAGCTCCAAGGAAGTGAATAAGTTTC11.2048192771084338No Hit
GTATTGGGCTGGTTGTGGTTGGGTGGTTTGGTTGTGTTGAATGGGTGGGG11.2048192771084338No Hit
GTACATATTAAGACCAGTTTTAATGACAAAGTCTTAGATGAAAACAAAAA11.2048192771084338No Hit
ATTTGTTCTTTTTGTTGTTTTGTTTTGGGGTTTTTGTATTGCTTTCGTAC11.2048192771084338No Hit
CAGTAATTAAACAAAATCCTCATAACAAATACGAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
TTACATGGGTATCTGTATTGGTGATAATACAAAAACGAGTCATTTCAGAA11.2048192771084338No Hit
CGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGCCTT11.2048192771084338No Hit
TCAACGCAGAGTACGGGTACGCCTTAACCTGTCTCTTATACACATCTGAT11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAACGG11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGGTTTAAAATTATAAATAATTTAAGTGAAAA11.2048192771084338No Hit
TCCCAGCACAATGGAGAAACAGTTACACAGAAATATTTTGCAATTACTAA11.2048192771084338No Hit
AAAAAAAAATAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11.2048192771084338No Hit
AAACAAATTCAAGATTATTAATAATTATTATTTTTAATATTATTTTAATT11.2048192771084338No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGACGAAAGA11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGGTCATTGATTGTGGCACAGTGGGAGAGGCT11.2048192771084338No Hit
GAAACAAAACCAAAAGCACAAGAAATACACAAAACAACAAACAACCTCAA11.2048192771084338No Hit
AACAAACACAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.2048192771084338No Hit
CCTCTCTGTATCTTCCAATGGCCCAGTCTGTGGCTTTTGGGCCTTTAGGT11.2048192771084338No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT11.2048192771084338No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11.2048192771084338No Hit
AGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTGGGGAAAGATCGGAAAA11.2048192771084338No Hit
AAAGATCATAAGTTAAAAGAAAAAAAACAAACAAACATAACACGAAATAC11.2048192771084338No Hit
ACCCTATAAGGATGAGATCGAGAAAGTATATAAGTCGAATCTGAAGAAGA11.2048192771084338No Hit
GAGTACGGGATACCAAAATACACTCATGGCGGAAATTTCAATAAAAAAAA11.2048192771084338No Hit
ACAGCTTTTATAGGCTTGTTGAACTGCATTTGATTCAGCAGCAAAAGCAG11.2048192771084338No Hit
AAATTCAAAAGCCATCATCCAAAAATAAAAAATTTATAATCAATTTTTTT11.2048192771084338No Hit
AAAAATCAGCAACACAACCAGCAGAATGACCAACAAGGTCAACCCAACCA11.2048192771084338No Hit
ATCAAAGCAGAGTACGGGAATAAAAAAAAAAAAAAAAAAAAAAATAAAAA11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAATAAAAAAAAAAAA11.2048192771084338No Hit
CCTCACTGTGGTCTGATTTCCAAACTCCCTGTAATTGACATGCAATCGCA11.2048192771084338No Hit
CTCCCATACCTTTGACTGCATCACTTGGATTTTTCAGTTCACCATCTGCA11.2048192771084338No Hit
AAAAAAGACATGCATTAAAAAAAATCAAAAAAAAACAAACAAAAAAAAAA11.2048192771084338No Hit
ATCAACGCAGAGTAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
CTTTGTCTCGATGAAATATATCTCTTGTAGTTTCCTGAAATTCAGGCACT11.2048192771084338No Hit
GCTTATTTAATTCAGCAATTCAATTTAAAGAAATACATCCAATTTAATCA11.2048192771084338No Hit
GTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAG11.2048192771084338No Hit
GCTTGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGG11.2048192771084338No Hit
TTATAAAACAAGAAGATGTACATAAAAAGCAAATATAAAAAATTAAAGAA11.2048192771084338No Hit
AATCAGTCTCTAGGATCACAAAGCTTAATTAAAACTTGTGTTTCTTTCTT11.2048192771084338No Hit
CCGCTCTGTGGGTACGGCTTTGCTCCGGTGGGGGGCATCGACTGCATCTC11.2048192771084338No Hit
CCCGTGTAGTCCTAGGCTTTACTCCTCAGATCGGAAGAGCGTCGTGTAGG11.2048192771084338Illumina Single End PCR Primer 1 (100% over 23bp)
AGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTACGGAATACGGCGT11.2048192771084338No Hit
GTATATAAATTAACGTCGACGACTACTATTGCGACTCAGCCCACGAGCCA11.2048192771084338No Hit
CTACTAGGGAGGCTGAGGCATGAGAATCGCTTTAACCCAGGAGGCAGAGG11.2048192771084338No Hit
AAATTTCACAAATAAATTTTCCAATTTCCACTAACAAATGTTATCATTCA11.2048192771084338No Hit
CAGAACTATCTTTTCGGTTGTGAACTAAAGTCCGACAAAGATTATCACTT11.2048192771084338No Hit
GTAATTAAGTGCATGTGGTCGATGCCTTGGCAGTAATATTCGATTAAAGA11.2048192771084338No Hit
CATATAACTTAATGGGCTGATCCACGTTCGATCGCCGCTACTGAGGGAAT11.2048192771084338No Hit
TCCTTGTGTTGTGTGTATTCAACTGACAGAGTTGAACCTTCCTTTAGACA11.2048192771084338No Hit
GTACGGGATTTTAGAACCGACAACTAGGTAATTTTCTAAGATCAAACTGC11.2048192771084338No Hit
AGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCA11.2048192771084338No Hit
GGGAGACAGAGAGGAGAGAGAGATGAAGGGAGACAGAGAGAGAGGAGAGA11.2048192771084338No Hit
CATCTAAACACCAACGGGCAAAACAGCGACATAAGACAAACCCCAAAAAC11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAATAAAAAAAAAAATAG11.2048192771084338No Hit
GACAGGTATCAACGCAGAGTACGGGAACAAAGTACAACTATAATGGTATG11.2048192771084338No Hit
GTTTGTTTTTATTTGATTAGTTTATTATGATTGGTTTTTTATTATCGGGT11.2048192771084338No Hit
GGCCTCCACGGCTAGGTCACCCTGCCGCTTCCATGGCCCCACGACGCAGC11.2048192771084338No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.2048192771084338No Hit
AAAAAAATTAAAAAAAAAAAAAAAAATATTAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGGAAAACGAGAATAAAAAAAAACTATTATAA11.2048192771084338No Hit
TATGTACACACACACACACACACACACACACATAGACACAACGGAATGTT11.2048192771084338No Hit
GAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAACGGGGATCAGTCC11.2048192771084338No Hit
GAGCAAGCAAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGA11.2048192771084338No Hit
GCTTCAGAGTACGGGCGCAAAATTGAATCTATGAATCCCAAAAAAAAAAA11.2048192771084338No Hit
AACCACAGCTCTGCAAGGCGGCCTTCAAGGAAACCGTGTTAATGTCGAGC11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTTAGTGCATGTGGTGTAAGC11.2048192771084338No Hit
TTCTATCTGAACTGGTGCTACCAGCGTTTGCTGCTTTATGGTTTGGTATC11.2048192771084338No Hit
CAAGAAGGGAGCCAAGCTGACTCCTGAGGAAGAAGAGATTTTAAACAAAA11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAGCACAAGCTGCGGTGCTACCGTT11.2048192771084338No Hit
GACTAGGTTCAAATCCTGGTTCCATGATTTCCCAGCTGTGTGATCTCTGG11.2048192771084338No Hit
GTATTCTAATTAATTTTTATTTTTTTTTTAGTTTTTTATTTGTGTTGTAA11.2048192771084338No Hit
AAGCTGGTCATAGCCAAAAACACAATGCCGCTGCACAAAAATCAGCAACA11.2048192771084338No Hit
GTTATCCGGGACGAGGACAATGTCTCGTGCGTAGATTGACTCGGGCGGTC11.2048192771084338No Hit
TTTAAATTATTTTTTTTTTTTAAGTTTTTATTTATTTTTTTTTAATAATG11.2048192771084338No Hit
GGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTA11.2048192771084338No Hit
ACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.2048192771084338No Hit
ATGATAGACTCTGTGTTCTACCATGTTCCTGATGTGACAGCTGCATGGCA11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAA50.085.03
CTATAAG50.085.04
ATAAGGA50.085.06
AGGATGA50.085.09
TATAAGG50.085.05
ACCCTAT50.085.01
AAGGATG50.085.08
CCCTATA50.085.02
TAAGGAT50.085.07
Produced by FastQC (version 0.11.5)