FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length61
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCTGCAGCTGCATTGGTCTTCTTAGTTTTGTTCTTATAGTCTCTCTTCTTCAACC11.2048192771084338No Hit
AACAAATCATCAACTAATAAAAGATGGAATCTATAACCAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
ACACTGACATCGTGATGGAGTAAACTCAAAAAAAAATAAAAAAAAAAAAAAAAAATATAAA11.2048192771084338No Hit
CTAAAACTCTGATCCAATTACATTACAAAATGCAAAATCATTCTGAGTAACAGTTTCTCCA11.2048192771084338No Hit
ATCAACGCAGAGTACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
GGACAAATAGTCTAGACATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
GAAAAAAAAAAAAAAAAAAAACAAAAAACAAAAAAAAAAAAGACCAAAAAAAAAAAAAAAA11.2048192771084338No Hit
AAGCCGAGTCGACCTTGTTTTTTTTTTTTTATTTTTTTATTCACGGTACCCGTAGTCTGCG11.2048192771084338No Hit
GTATCAACTCAGCGTACTGGCAAAGCTACAATACAGAGAAAAAGTAACCGATAAAGGTGAA11.2048192771084338No Hit
AAGTAGTACTGAATATTTTTGTTTTGTTATAACGATTAAATAATTTATTAGTAGTAGAGGT11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGCACACCCAAAAAAAATATCAAACAAAAAACGAAAATAAAAA11.2048192771084338No Hit
GAGTACGGGAGAAAAAAAAAAGACAAACAAAAATCAAAAACTAAAAAAAAAAAAAAAATAA11.2048192771084338No Hit
CAAAAAGTGTCAGGGATAAATCAAAATTAACTACAAGCACCCCACCTGATAAAAAAACAAA11.2048192771084338No Hit
GCGGGGGGGCGGGTTGGGGGGGGGGGGTGTGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGG11.2048192771084338No Hit
AAACATACAACACTAATAATATTTATAAAAATATATTTATTGTGATCATGACTTTGTCAAA11.2048192771084338No Hit
GAGCTCACACGTCATTTTGGTATTTTTTTTTTTTTTTTTTATTTGGTTGGAGAGGGGTGGG11.2048192771084338No Hit
CTTAATAACGAAGTGGATATATCACTTCCTTCTCTCTCTTCTTCAGAATCGCCTTATACAT11.2048192771084338No Hit
CGGTCAGACGACGGCATGAGACATACCTGAGATATGAGCTGGCGCGCAAGCACAATGCCAC11.2048192771084338No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAAACAGGGAAAA11.2048192771084338No Hit
TATCATATATTTCCTGATTAGTATCAGGTAAATATCTAGACTCCTATCCTATCACAGGATA11.2048192771084338No Hit
GAGCGCACCTTCGTACTCCTCCGTTACTCTTTAGTATGAGACCGCCCCAGTCAAACTACCA11.2048192771084338No Hit
GAGCCTCAAAGTTTGAGCACCAGAAAGACAACCAAACAACATCAGACTAAACTGCAACTTC11.2048192771084338No Hit
GGGTTGGGTGGGGGAGGCTGGTGGGGGGGGGTGGGGTGGAGCGGGGGGTGGGGGGAGTTGG11.2048192771084338No Hit
GTCTAAGATCAGAAAAAACAAACTAAAGATGGTGAACAAAAAAATCCAATTGATGCAGACA11.2048192771084338No Hit
CCAATAAACTAAATTATTATATATCATAAAAAATATGCAAATAGATTAATTTAAAAAAATT11.2048192771084338No Hit
AAACAAAACAAAAAAAACCAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAA11.2048192771084338No Hit
CTCTTTATGCCATTAAGCTGTCAAAGCAGGAAAATGGTAGAACACAGAGTCTATCATCTGT11.2048192771084338No Hit
GATTACAGCAAAAACAAATAAAAATTAAACAAAAACAACCAAAATAATCAACAAAAAAAAA11.2048192771084338No Hit
TCGTAATTCATTGCCTCTGCTTCAACAATGTGAAACTCATCCTTTGCACCAGCCCCTAAAC11.2048192771084338No Hit
ACTTCTTCCTTCACCAACATGCAAGTTCTTTCCTTCCCTGCCAGCCAGATAGATAGACAGA11.2048192771084338No Hit
CAGCACGTACACGGTCACCTACTTGTACAGGCGATAAAGTACGGCAATTCCATTCATCAGA11.2048192771084338No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATCCTGCGCCGACAATGTAGCGGG11.2048192771084338No Hit
CAACTAAAAAGATGTTGAAAAACAACACGTAAACATATCACAATGAATAATAAAACGATTG11.2048192771084338No Hit
GTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAATATAAAAA11.2048192771084338No Hit
ATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGCGTTTTTAGGGGGG11.2048192771084338No Hit
GGATTAGTCTACAGTGCTGCGGCAACAGGGCTGGGACAAGTTTTTTTTGATCAACAAGCCA11.2048192771084338No Hit
ATGTGGTTCGATTTTTTTGTGTGTCATCTCCGATTTGTTTGAGCGGTGGTTTGTAGTTCTG11.2048192771084338No Hit
AATAATAAAAACAAACAATTTGTTCAATCAGACATCATCTACTTAAAACAAAAAAAAATGA11.2048192771084338No Hit
TATAAAAAAAGAAATAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGGAACTGGGTGA11.2048192771084338No Hit
CCCAAAAAAAAAATAAGACATGAGCAAACGCTGTTACACCAAACAATATAAAAAAAAAGGT11.2048192771084338No Hit
TCATCATATTTCTTCTGAATTTTTTTAGATCGTTTTTTGTTTAAAATCTCTTCTTCCTCAG11.2048192771084338No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTG11.2048192771084338No Hit
AACTAACCATAAAAACAATTAGACACAAAAGCTACTGAAACTACGAATACAACAACAAAAA11.2048192771084338No Hit
AAGGAATCAGCACACAATCGAGAAATATTGTTTTTTTTTTTTTTTTTTTTTATAAGAAAAA11.2048192771084338No Hit
GTGCAGCAATTTGCCATTCACATGCTTCTGGCAGTATGAGCAAATTAGCTGTACTGAATGC11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
AAGGAAAGGATAAAAAAAGTAAAAGTAAATATTAAATTATTAAGAAGCCTGTTTACCAAAA11.2048192771084338No Hit
GTTTCATCCTACCACTCCCAATTAAACTTTCCATTTTCGTCTGCGTTTATTAAATTCGTTA11.2048192771084338No Hit
CTCAAATCACATAATCGACTGATAAAATTTATATATAATATAATTATTTAATCTCAATTGA11.2048192771084338No Hit
CTTTTAAACACGGTTTCAATATTTGATCTTCCTTACCCACGTACTTTCCTCTTACTACATC11.2048192771084338No Hit
GGATTGGCGCTGATTACAACATCAACAAGCAAGATCGCTTCTACGGTGTTGTTGGTCAAAA11.2048192771084338No Hit
CACTTAGTAACTGTTTTCAAGGTTCATCTGTCTTGTATCCTGTATCCAATTCGTATCCCTT11.2048192771084338No Hit
GTGGTATCAACGCAGAGTCGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTATTATCACAGA11.2048192771084338No Hit
GTGGTATCACCGCAGAGGCGAGGTTTTTTTTTTTTTAGTATTTTTTTTTTTAAAAATTTAA11.2048192771084338No Hit
CTAAACGCAGAATACCGGTGACAAACGGAACCAAAAGAGCAATTTCATACAAATAGAAACC11.2048192771084338No Hit
CGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATT11.2048192771084338No Hit
GTTATTGGCCTTAGGTCTCTAAATAGTGTTTTGGAAAATACATTATTTTTATTCTCTGCGT11.2048192771084338No Hit
CATATAAACACATAGGAGTTGATGATAATGATGGTCAAGTTTTAGATAACACAACAAGGGT11.2048192771084338No Hit
GGGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTGGGGTCTTGATTGACAGTTTT11.2048192771084338No Hit
GGTTACTGAGATGTTTCACTTCCCATCGATCTCCTCGCATGACTATGTATTCATCATGCGA11.2048192771084338No Hit
GAGTGCAGTGGTGCAGTCTTGGCTCACTGCAACCTCTGCCTCCTGGGGTAAAGAGATTCTC11.2048192771084338No Hit
CTATGACGCTCAACCCCTTGATATTGGTAAGCCTCTCCCACTGTGCCACAATCAATGACCC11.2048192771084338No Hit
TCGCTGCGAGGGCGAATTTTATTTGTGTTATTTTTATTTGTTAGGGAGTTGTTGGTTGGGG11.2048192771084338No Hit
ACGCATACTCGACAATTATTTTTTAATATTGTTTTTTTTATTAGATGGAGATAAAAGATGT11.2048192771084338No Hit
GGAAAAAACAAAGAGAACGGTTATACTGTGATCATGAGATTTAACAATCGGGAAAACCACA11.2048192771084338No Hit
ATATCGTGTCCAGGACATAGAAAGAGCGCAATAAATAGTAACAATTATTTTAGGCAGTGAA11.2048192771084338No Hit
CATATAAACACTAATTCAACAAATAATAGGCTTAATTACATTATATACCATATAACAATAA11.2048192771084338No Hit
GAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTGCAGTTTGAAGGGGTGGGTAGAGGGGGT11.2048192771084338No Hit
CTTTGCTCACTGTGTCTCTCTTGTTCCTCCTTTTTCTCTAAATTCCTTCTTACTCCCTCCA11.2048192771084338No Hit
GAAGTGAATGGATCGCAGCACTCACTGACCTGTGCTTTTGAGGACCCAGATGTCAACATCA11.2048192771084338No Hit
TGATAGGACATAGTGGAAGTGGGCTACAACGAAGTACGTGTCGTGTAGTACGATGTCTAGT11.2048192771084338No Hit
ATAACAAAAAAACAACAAAACAAACAAACAAAAACAAAAAAACCAAAAAACAAAAAAAAAA11.2048192771084338No Hit
CTACGAGCACGAAACAAAATTAAAAAACAGAACAAAACAAAAAACAAGCCAAAGTTCGATA11.2048192771084338No Hit
AGCGGGAACTGCTTCTGAAATGACTCGTTTTTGTATTATCACCAATACAGATACCCATGTA11.2048192771084338No Hit
GACCAAACGACTTAAATGGTAAACACTATTCAATACACACTAAATATTCACTAAAAAAATA11.2048192771084338No Hit
ACTATACTCCAGAATTTTTTGTTTTGATCTAAGACATTTGAATAAAAACAGGTATACATAA11.2048192771084338No Hit
CCAAACTGCTGAATGAAAAGAAAAGTTAAACTCTGTGAGTTGAACGCACACATCACACAGC11.2048192771084338No Hit
CCAATCAACGCATAGACGACGTTTTTTTTTTTTTTTTTTTTTTATTTATAAAGCATTTAAA11.2048192771084338No Hit
CTTATCGAGATTGCTAAGTGCTTTATAGACAGCGTAAAAAATTATCAAGATTTTAAAAGAT11.2048192771084338No Hit
GTTTTAATATCCCTTGCGTCTAAGTGTTTGGTAGAGGTTTTGCCAAATAAGTTGCTGGGAA11.2048192771084338No Hit
ATCAACGCAGAGTCGAGGGTTTATTTTTTTTTTTTTTTTTTTTTTAAAAATTATGATAGCG11.2048192771084338No Hit
TCACTGGACTCTTCACTGCTGCTACTCTCTGATCCTTTCGCTGCAGCCTACCCAGCAGGCC11.2048192771084338No Hit
ATTTTAGTGGATCATCTGAGTTGATGTGGCAAGAATACCCTTCAACACCAGATGAAGCATT11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGA50.055.041
TCCTTCT50.055.027
GCCTTAT50.055.051
TATACAT50.055.055
TATATCA50.055.018
CGCCTTA50.055.050
GATATAT50.055.016
AACGAAG50.055.07
CTCTCTT50.055.034
CTCTCTC50.055.032
GAAGTGG50.055.010
TAACGAA50.055.06
CTCTTCT50.055.036
AGTGGAT50.055.012
CGAAGTG50.055.09
TTATACA50.055.054
GGATATA50.055.015
ATAACGA50.055.05
AGAATCG50.055.045
TTCCTTC50.055.026
Produced by FastQC (version 0.11.5)