FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005413880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779765
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102190.5741769278528345No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT91340.5132138231732841No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA70710.39729964349225877No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.34999002677319757No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC59690.3353813565274067No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT58870.33077400668065726No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG51980.2920610305293115No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA49240.276665739577978No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT48140.2704851483201434No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA47990.26964234042134777No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT46180.2594724584425472No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC46010.2585172761572455No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA41870.23525577815048615No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA40670.22851331496012117No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA40140.22553539371770992No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG39630.2226698468618048No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT37810.2124437776897512No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG36700.20620699923866354No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.20227389571095059No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC35060.19699229954516467No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT34570.19423912707576563No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT34310.19277826005118653No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG33940.190699333900824No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT33930.19064314670757093No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT33930.19064314670757093No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC33410.18772141265841277No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC32280.18137225982081906No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA31470.17682109716732267No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA31430.1765963483943105No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA31100.1747421710169601No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.17035956994322285No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG29780.1673254615075586No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG29460.16552747132346124No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC28640.1609201214767118No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG28600.16069537270369966No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA28470.1599649391914101No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT26410.1483903773812835No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG25200.14159172699766542No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT25180.14147935261115935No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.1326017760771787No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG22390.12580312569356067No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG22190.12467938182849983No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG21940.12327470199717377No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG21840.12271283006464336No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT21100.11855497776391828No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.11759979547861656No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG20130.11310482001837321No Hit
TTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTATCGCATGATGAA19900.11181251457355326No Hit
CCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAAC19820.1113630170275289No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.11108208106126372No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC19130.10748610069306902No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT19120.107429913499816No Hit
CAGTAGCGGCGAGCGAACGGGGATCAGCCCATTAAGTTATATGTGTTTTA18840.10585667208873081No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.10248544049354831No Hit
GATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC18230.10242925330029526No Hit
GTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA17860.10035032714993271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)