FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177077
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22810.19378511346326535No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19220.16328583431670146No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT17160.14578485519638903No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC16480.14000783296249947No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC13930.1183439995854137No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT12860.10925368518796985No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA12580.10687491132695653No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA12470.10594039302441556No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT12370.1050908309311965No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT12220.10381648779136793No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA12100.10279701327950508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28500.037.991552
GTGGTAT29250.037.2195471
GTATCAA110850.025.9912621
AGAGTAC74900.024.56474511
CAGAGTA76350.024.2052610
CGGGATC4650.023.65783517
GGTATCA57200.023.118031
TATCAAC125000.022.9522972
GACAGGT4200.022.2551961
AACGCAG133850.021.3338766
TCAACGC134550.021.1802334
AGTACGG85200.021.14314813
GAGTACG86350.021.11636212
ATCAACG135250.021.1112523
CAACGCA136650.020.8949625
CGGGGTC5750.020.56688917
GTCGAGG19650.020.4290168
ACGGGAT11350.020.11176716
ACGGGCT2500.019.80160716
TACGCAG700.00499582319.6294274
Produced by FastQC (version 0.11.5)