FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594374
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA12010.20206132838919605No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10440.17564698321259006No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10300.17329156389747868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9740.16386988663703325No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT9380.15781309411246117No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC9000.15141981311430178No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC8410.14149340314347533No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8400.14132515890668165No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA8260.13896973959157027No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT7830.13173523740944254No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA7560.1271926430160135No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT7520.1265196660688388No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA7420.12483722370090214No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6940.11676150033480602No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG6690.1125553944149643No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA6690.1125553944149643No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG6580.11070470781023396No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA6110.10279722868093152No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC5990.10077829783940752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTG257.8539364E-443.9993979
GACAGGT2500.031.8941921
GGATCCA400.00787065227.4996219
GCTTGTA2600.027.4949931
ACAGCCG400.00787712127.4949931
GTATAGC400.00787712127.4949931
GCTTGGC904.7199137E-827.4949911
TTGTACG606.3386855E-527.4834233
TGGTATC28500.026.9093552
GTGGTAT28950.026.402791
CGATCGT1351.6007107E-1024.44822547
AGAGTAC55650.024.2196211
GTCCGGA1251.7407729E-924.19966755
GTATCAA88200.024.128261
CAGAGTA56450.023.97383710
GGTACGG1052.1071901E-723.5572223
GACAGGC1653.6379788E-1223.3290841
GCTTCGT952.3165176E-623.1536791
CCATCGG1202.9865987E-822.9221426
CCGATCG1202.9893272E-822.9202146
Produced by FastQC (version 0.11.5)