FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275442
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.2886058323310664No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30640.24023044560238724No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.1736652862301853No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.12732840850465957No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.11501895029331008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15450.051.4390642
GTGGTAT15300.050.584251
GTATCAA58300.039.6065181
GGTATCA32050.037.1505621
TATCAAC68150.034.485712
TCAACGC69300.033.7907834
ATCAACG70300.033.3705753
CAACGCA72000.032.523635
AACGCAG75050.031.3717656
ACGCAGA84150.027.9287137
CGCAGAG84700.027.6982678
GCAGAGT89300.026.5570419
CAGAGTA46400.023.72349710-11
GACAGGT6700.023.4834731
AGCGTCA1852.9245712E-822.9724963
GTCGAGG9600.022.1358248
AGTCGAG13850.021.4796987
AGAGTAC43550.020.44526910-11
GAGTACG54600.019.96602812-13
GCGTCAG2201.8245373E-719.317784
Produced by FastQC (version 0.11.5)