FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275442
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30400.2383487449840918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19550.042.1887131
TGGTATC20150.040.7800372
GTATCAA75750.026.7489431
AGAGTAC47750.025.40337811
CAGAGTA50500.024.4003410
TATCAAC83700.023.9032752
GGTATCA38100.022.8025781
TCAACGC88250.022.4822584
ATCAACG89450.022.2728123
CAACGCA90650.021.9173435
CGGGGGA2900.021.81418817
AGTACGG55650.021.69914613
AACGCAG92800.021.439176
GCCGACG903.9954728E-521.38930155
GAGTACG57700.020.92820712
ACGGGGG8000.019.94257716
CCGGTAT700.004967873519.64863249
ACGGGAT11200.019.64786116
TACGGGG25300.019.570215
TCGCCTA1008.971496E-519.25490213
Produced by FastQC (version 0.11.5)