FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956104
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25570.2674395254072779No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24200.25311054027595326No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.16431266891467874No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.14172098432806474No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC13340.1395245705488106No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12840.13429501393153884No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA11090.11599156577108767No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC10920.11421351652121527No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10470.10950691556567069No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10460.10940232443332523No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.10898395990394351No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.10626459046296219No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT10040.10500949687481696No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9860.10312685649259913No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG9770.10218553630149022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23800.035.178012
GTGGTAT23700.034.6308861
GTATCAA70300.034.480551
AACGCAG73850.033.262846
GTCGAGG8900.031.5163528
TCAACGC79900.030.6378084
CAACGCA81300.030.2147675
ATCAACG81600.030.1036833
GACAGGT3750.029.4846971
TATCAAC83250.029.4559862
ACGCAGA83550.029.4519547
CGCAGAG84250.029.2072478
GCTTGCC1503.1159288E-928.3506721
GGTATCA43100.028.0217781
GCAGAGT92500.026.6482249
GACAGGA4050.026.2506221
GACAGGG3250.026.1698511
CGGGCCG1001.0558533E-425.4995926
GCTTGTA5200.025.3520451
GTACAGA1551.3389945E-724.692521
Produced by FastQC (version 0.11.5)