FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956104
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17840.18659058010425644No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA11860.12404508296168616No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC11630.12163948691774117No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT10920.11421351652121527No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG10590.11076200915381591No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9680.10124421611038129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCC350.00411574131.4028684
AGTGCCG453.8133137E-430.5305655
TGGTATC23600.029.691232
GTGGTAT24850.028.5443781
TGCCGAC759.748739E-625.66715655
CCGCGTC550.0012273924.996559
GTATCAA81450.024.843571
AGAGTAC57400.023.72057711
ACAGGTA3950.023.6651441
CAGAGTA58900.023.06978610
CGTGGCA600.002036913322.91350443
AACGCAG87600.022.8002436
TATCAAC91500.022.1032832
AGTCCGG1003.611991E-622.00157254
GCTTGTC1506.002665E-1021.9946611
TCAACGC92850.021.6328034
ATCAACG93900.021.4786913
AGACAGG1801.0913936E-1121.3836981
CAACGCA94500.021.2550895
GGTATCA45000.020.5894491
Produced by FastQC (version 0.11.5)