FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722404
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33020.19170879770367463No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT24370.14148829194544368No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT22290.12941214720820435No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC20350.11814881990520225No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC17510.10166023766781777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT51000.037.25131
TGGTATC51350.036.7147562
GTATCAA174250.025.4188771
AGAGTAC108450.024.5488311
CAGAGTA110150.024.3447410
TATCAAC191200.022.96922
AACGCAG204250.021.381176
GGTATCA92050.021.1966083
ATCAACG206200.021.1771263
TCAACGC206750.021.0809154
AGTACGG126450.020.7715813
CAACGCA210650.020.7688945
GAGTACG127550.020.67869612
CGGGGTC6750.020.37282617
GACAGGT6100.020.2822571
TTAAACG3800.019.5412622
ACGGGAT15650.019.15566316
GCTTGTA3800.018.8114951
ACGCAGA234150.018.663167
CGCAGAG235600.018.4948048
Produced by FastQC (version 0.11.5)