FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659480
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14020.2125917389458361No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG12910.19576029599078063No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT11070.16785952568690482No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT9180.13920058227694548No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8180.12403712015527385No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7470.113271062048887No Hit
ACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCA6890.10447625401831745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT202.612096E-455.00303340
TCCGGTA202.612096E-455.00303339
TGGTATC21400.044.8369982
GTGGTAT22100.043.9210971
GCCTCCT358.6608794E-539.2878816
GACAGTC350.004100314331.4255371
CTCCTCG453.794267E-430.55492221
CTGTCCG400.00786570527.50360554
GTATCAA79550.026.7887381
CGGGCAC759.740028E-625.66808317
AGAGTAC51550.025.55427411
CCTCGCT651.0878652E-425.38601723
CGCCAGG651.09067274E-425.3763896
CAGAGTA52250.025.21191610
GCCGACG550.00122560525.00137955
TCCCGTG550.001227232424.995697
TAACTAG550.001228318324.9918985
AGACAGG1900.024.6028881
GGTATCA40600.024.3763353
GACAGGT1700.024.2623631
Produced by FastQC (version 0.11.5)