FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322413
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26370.19940820303490664No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT17300.13082146046658644No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14390.10881623214532828No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC13650.10322040088837602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29000.040.837752
GTGGTAT29700.039.888821
GTATCAA107000.026.1770551
CGGGATA2450.025.81797817
AGAGTAC68850.025.12696811
CAGAGTA71400.024.4221810
TATCAAC117650.023.6358432
GGTATCA56050.022.4021783
TCAACGC128400.021.6973254
AGTACGG79050.021.64122613
ATCAACG128650.021.591913
GAGTACG80100.021.46055212
AACGCAG130350.021.3540846
CAACGCA130700.021.2742675
TACGGGG26450.020.69287315
ACGGGAT11350.020.59759116
ACGCAGA148500.018.8202927
GTACGGG89500.018.80709614
CGCAGAG148400.018.8058978
ACGGGGC4100.018.78311216
Produced by FastQC (version 0.11.5)