FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928454
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.3078235432234661No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.2855284160550765No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.17103701421933665No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.16145118659621263No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC13510.1455107092004558No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12910.13904835349947334No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC11200.12063063975167321No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA11010.11858422711302875No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.1179379915429305No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10640.11459910776408955No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.11449140183573984No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10170.10953692913165326No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT9670.10415163271416784No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9400.10124357264872574No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9390.10113586672037603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21150.040.9918672
GTGGTAT22200.038.6951981
GTATCAA71550.036.3747861
GACAGGT3800.034.6925931
AACGCAG81900.032.5358056
TATCAAC82950.032.1239622
ATCAACG86100.031.1461323
TCAACGC86200.031.114
CAACGCA87450.030.568125
ACGCAGA90900.029.361197
GGTACGG1452.1846063E-929.3096053
CGCAGAG91650.029.120928
GGTATCA41400.028.967261
GTCGAGG9950.027.7631158
GCAGAGT100500.026.556549
GGCACCG4550.026.1531839
GACAGGA3600.025.988361
GCTTGTA5400.025.2008341
CAGAGTA59850.023.21996310-11
TTGTACG1652.464276E-723.181233
Produced by FastQC (version 0.11.5)