FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527162
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3700.049.3908962
GTGGTAT4150.044.0561521
GTCGAGG1650.038.6355868
GTATCAA13350.035.0346371
GTTACCG500.00278789833.9993179
TATCCCG500.00278789833.9993175
TCAACGC14850.031.194664
AGTCGAG2200.030.908477
CAACGCA15050.030.7801135
ATCAACG15150.030.5769443
TATCAAC15700.029.5057752
GGTATCA8100.029.3960691
GTACTAG755.8857084E-428.3462071
AACGCAG16700.028.2479346
TCGAGGT2351.8189894E-1225.318649
CGCAGAG18250.025.150188
ACGCAGA18600.024.9054157
AGCACCG900.001710040723.6106385
GCAGAGT19650.023.5745939
TTATACT1353.1699925E-522.0365944
Produced by FastQC (version 0.11.5)