FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527162
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9810.18609080320660443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4650.039.020342
GGACCGT300.001928463936.6519976
GTGGTAT5250.035.0978431
AGACGGT606.308184E-527.5020410
CGGGATA400.007866478527.5020417
GTATCAA17100.024.767921
AGAGTAC13350.023.89690411
GGGTAGT701.7979163E-423.573181
TATCAAC17950.023.4329552
ACGGGAT1950.022.56577916
CAGAGTA14150.022.54584110
GGTATCA10050.022.1658251
TCAACGC19100.022.019984
GTGCTAA1003.6068122E-622.0016331
ATCAACG19150.021.9624883
CAACGCA19400.021.8211615
ACGGGGT1801.0913936E-1121.39047816
TTTCCGT650.003236057521.15541627
TACGGGG7550.020.76312815
AGTACGG15150.020.69460513
Produced by FastQC (version 0.11.5)