FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989025
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT153221.5492024974090646No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.3124289072571472No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT25680.25964965496322134RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.24832537094613383No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT18580.1878617830691843RNA PCR Primer, Index 29 (96% over 25bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.16207881499456536No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC13420.13568918884760245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44100.045.1096881
GTGGTAT9550.044.9552651
TATCAAC47800.041.791562
TGGTATC9950.041.4349982
GGTATCA20200.039.7715951
TCAACGC51600.038.9570584
ATCAACG51750.038.8441433
CAACGCA52250.038.5537645
AACGCAG54300.037.17656
CGCAGAG58450.034.8277748
ACGCAGA58400.034.7813037
GCAGAGT61550.033.2117469
TTAGTAC1457.0655005E-826.3784483
CAGAGTA44350.025.97002210-11
AGAGTAC42950.024.09530810-11
GAGTACG48650.023.63094512-13
ACGTTCG450.001712328623.60914652-53
GCTTGAA34600.022.41617656-57
TATTCCG950.002343322422.367695
AGGGGGG32250.022.14552970-71
Produced by FastQC (version 0.11.5)