FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989025
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271702.7471499709309675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8500.034.6055832
GTGGTAT8600.034.538571
GTATCAA41900.030.3910711
TATCAAC46700.027.6081492
GGTATCA19150.027.5747431
TCAACGC49300.025.9835414
CAACGCA49750.025.803775
ATCAACG50250.025.436323
AGAGTAC46200.025.35471711
AACGCAG51000.025.1713246
ACACCGT550.0012292924.9900256
CAGAGTA47400.024.65356810
CGGGATA2950.024.23864717
ACGCAGA53300.024.1379287
CGCAGAG53400.024.042468
GCAGAGT56100.022.8888119
AGTACGG50750.022.59618213
ACGGGAT10150.022.48894716
GAGTACG51650.022.200212
TACGGGC6600.021.66788315
Produced by FastQC (version 0.11.5)