FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840496
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15720.18703241895261846No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC11170.13289771753821553No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG9890.11766861472273515No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9760.11612190896803792No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT9750.11600293160229198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19500.031.2861522
GTGGTAT20300.030.3383921
CGCCTAT1004.300091E-927.50726536
GACAGGG2500.026.3944021
GTATCAA66550.026.2754171
CTAGATA550.001231210224.98284
AGAGTAC51900.023.42203911
GACAGGT2850.023.1529831
CAGAGTA53000.022.93455710
TATCAAC77050.022.648262
AACGCAG78200.022.4922476
GAGACCG1003.6256388E-621.99140531
TCAACGC79750.021.9159454
ATCAACG79800.021.8677773
GCACCGC3150.021.82596210
CAACGCA81450.021.4922625
GGTAACG903.9840204E-521.39581552
GCCAGGT1459.677024E-920.8626117
GCTTGCC1351.0443182E-720.3660491
TCTATCG1351.0462827E-720.36241331
Produced by FastQC (version 0.11.5)