FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865261
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.20629613492345084No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.19011604590984685No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.12736041494993997No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.114185199610291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11550.039.0284231
TGGTATC11900.038.2113762
GTATCAA38750.037.8620871
TATCAAC43350.034.213082
TCAACGC45200.033.0008054
ATCAACG45300.032.927963
AACGCAG44850.032.8793226
CAACGCA46550.031.9524545
ACGCAGA50650.029.0303657
CGCAGAG51700.028.5229728
GGTATCA22000.028.0286671
GTCGAGG6600.027.0433948
GCAGAGT56650.026.1071999
TCGAGGT7700.022.6294549
CAGAGTA34350.022.02288410-11
GTATATG2900.021.9963381
AGTCGAG9750.020.485627
GCTTGTA4050.019.9505881
AGTACGG42050.019.8082432
AGAGTAC33000.019.38222110-11
Produced by FastQC (version 0.11.5)