FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453241
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26620.18317677522172854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22100.042.9160271
TGGTATC23400.040.5206452
GTATCAA90150.027.5978681
GACGCGT507.027673E-427.50344312
AGAGTAC56100.025.9346211
TATCAAC98550.025.1549222
CAGAGTA58700.024.97331610
ATCAACG105750.023.3098963
TCAACGC105650.023.2539254
GGTATCA44650.023.0888611
CAACGCA107650.022.8474255
AACGCAG108750.022.6163256
AGTACGG65350.021.96908613
GAGTACG68100.021.32425512
TACGGGG30000.020.26156815
ACGCAGA121950.020.1492427
CGCAGAG123850.019.844918
ACCGCGG1251.124261E-619.799079
GTACGGG73150.019.43919214
GCCGTTA1008.979143E-519.25307315
Produced by FastQC (version 0.11.5)