FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718158
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48540.6758958335073897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6750.035.8759881
TGGTATC7000.035.353832
GTATCAA30550.028.2841681
TATCAAC35000.024.8262442
GGTATCA15200.023.535571
TCAACGC37250.022.7956474
ATCAACG37950.022.5971283
GTGCTAA1103.2621392E-722.5151481
AGAGTAC34400.022.45842711
CAACGCA38000.022.3472885
AACGCAG37850.022.145426
CAGAGTA35400.021.89862410
CGCAGAG38900.021.6198188
ACGCAGA39100.021.5077327
CCGAAAT650.003235105721.1577647
TCCGTTA650.003237721721.15481213
GCAGAGT40650.020.6905179
AGTACGG37300.020.27572413
GAGTACG38550.019.82676512
TACGGGG16850.019.42495715
Produced by FastQC (version 0.11.5)