FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560553
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT73710.47233256416155045No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.3675620116715036No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40030.2565116340169158No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT37020.23722359958296835No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.10643662855410871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19850.050.962482
GTGGTAT20600.049.324451
GTATCAA70200.038.516921
TATCAAC77050.035.3054282
GGTATCA40200.034.5822531
TCAACGC81450.033.448244
ATCAACG83450.032.6466033
CAACGCA84550.032.3224035
AACGCAG86500.031.6428816
ACGCAGA95500.028.656247
CGCAGAG96050.028.4479068
GCAGAGT104200.026.34529
CAGAGTA59000.024.81269510-11
ACGTACG552.0168854E-423.18200134-35
GAGTACG67500.021.71960812-13
AGAGTAC54850.021.53797510-11
GTACGGG71050.020.51477414-15
GTATATA4800.019.4855631
AGTACGG61750.019.09689312-13
ACGGGGA10500.018.61680816-17
Produced by FastQC (version 0.11.5)