FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560553
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77140.49431195223744406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13200.034.5982131
TGGTATC13350.033.572922
GTATCAA59000.029.2371541
TATCAAC65550.026.3013292
GGTATCA29150.024.916381
TCAACGC68150.024.805784
ATCAACG68450.024.7033963
AGAGTAC63850.024.2877411
CAACGCA70500.024.211315
AACGCAG71350.023.9606346
CAGAGTA65900.023.65282610
ACGCAGA74900.022.7523217
CGCAGAG75200.022.516078
AGTACGG70450.021.47285713
GCAGAGT80450.021.1861029
TACGGGG31450.021.07679215
GAGTACG75950.020.06016212
ACGGGGA12100.019.54885116
GTACGGG77950.019.30097814
TACGGGA34750.018.20460115
Produced by FastQC (version 0.11.5)