FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022892
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35080.1734150908699031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT31950.043.7206571
TGGTATC31800.043.6448022
CGCGTCT400.00787320927.50094218
GTATCAA131000.026.9098031
AGAGTAC87350.024.62140811
TATCAAC145100.024.0549562
CAGAGTA90750.023.69895410
GGTATCA64500.023.6606711
TCAACGC152800.022.6263684
ATCAACG154350.022.4881783
ACGCGTA1354.256435E-922.40706433
GACAGGT7800.022.2095491
CAACGCA155400.022.1770655
AACGCAG157350.021.9726376
CACGCGT1406.488335E-921.60307132
TCACGCG1406.491973E-921.60200331
AGTACGG100200.021.35408213
GAGTACG103650.020.7229112
ACGCAGA175900.019.6593427
CGCAGAG178100.019.4049038
Produced by FastQC (version 0.11.5)