FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171294
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48210.4115960638405046No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.33731923838079936No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.23742971448671302No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.17655686787433386No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.15700584140275628No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.11926979904276808No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.1029630477062121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16850.060.7834472
GTGGTAT17250.059.1503371
GTATCAA69450.041.5042461
GGTATCA35000.038.1414071
TATCAAC76550.037.75132
TCAACGC81350.035.367094
ATCAACG81450.035.271493
CAACGCA84050.034.281535
AACGCAG88200.032.7166946
CGCAGAG97800.029.5064968
ACGCAGA98600.029.2670967
CGGATCG600.006804384328.33449
GCAGAGT104000.027.8712439
GTCGAGG13700.026.9889168
CAGAGTA51350.024.9971410-11
GACAGGC3600.024.8000131
TCGAGGT14700.024.5757569
GACAGGT6800.023.1327841
AGAGTAC48600.022.47608610-11
TATACCG950.002343782722.3673535
Produced by FastQC (version 0.11.5)