FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171294
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32050.2736289949406383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22050.044.5095631
TGGTATC22350.043.4052732
GTATCAA85650.027.0257931
AGAGTAC55400.024.72075311
CAGAGTA55850.024.47233210
TATCAAC94900.024.1805172
GGTATCA43750.024.0665841
ATCAACG103050.022.2395653
TCAACGC103050.022.1852864
GACAGGT5150.021.8861981
CAACGCA104500.021.8774535
AACGCAG107350.021.3505636
AGTACGG64500.021.02163513
GAGTACG65650.020.69440512
GACAGGC2550.020.483641
CGCGAAG700.004973002719.64496429
TACGGGG25450.019.45198615
ACGGGGC4450.019.15935916
CGCAGAG120800.018.9579038
ACGCAGA120950.018.9295437
Produced by FastQC (version 0.11.5)