FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080628
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52420.4850883005067424No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.36330726207353503No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT31650.29288524820752376No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.24328446051740285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13600.056.876952
GTGGTAT13700.055.5362431
GTATCAA49950.039.9099661
TATCAAC54900.036.7726632
GGTATCA27250.035.5641441
ATCAACG59300.033.8976863
TCAACGC59850.033.7281954
CAACGCA60950.033.1892055
AACGCAG63150.032.0329746
ACGCAGA69650.029.0395017
CGCAGAG70700.028.6683248
GCAGAGT75200.027.065819
CAGAGTA42250.025.14297310-11
TATGTCG1452.0790994E-623.4469095
GAGTACG48050.021.97537612-13
GTACTGG4850.021.0335961
AGAGTAC41400.020.62999210-11
GTACGGG52550.019.89141714-15
ACGGGAT9450.019.78491816-17
GACAGGT5400.019.6784211
Produced by FastQC (version 0.11.5)