FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718353
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.20158838143268584No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.19867861842124407No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.13320895066380423No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.10963987027112589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24700.046.6290932
GTGGTAT25350.045.9564821
GTATCAA81900.039.6109121
TATCAAC92800.035.4468462
TCAACGC100750.032.4810684
ATCAACG101400.032.3147663
AACGCAG102150.031.9942996
CAACGCA103400.031.6897245
GGTATCA46100.031.4505061
CGCAGAG112900.028.9478978
ACGCAGA113600.028.769527
GCAGAGT121100.027.057959
TTGTACG2051.2914825E-1024.8778023
GCTTGTA6500.024.8567561
GCGTCAG4150.023.5539844
GTCGAGG11400.023.486618
CGTCAGA4350.023.4480445
AGCGTCA4250.022.9997733
CAGAGTA75900.022.6776410-11
AGAGTAC73600.020.12378110-11
Produced by FastQC (version 0.11.5)