FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046656
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23610.22557554726672374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21200.042.9109572
GTGGTAT21800.041.8761
GTATCAA84500.027.854841
CAGAGTA55400.025.81676310
AGAGTAC55350.025.59162111
TATCAAC92400.025.4016532
ATCAACG99450.023.4892673
TCAACGC99700.023.3201074
CAACGCA101550.023.003525
CAAGTCG852.5665851E-522.65099737
GTTAGAC852.5827507E-522.6325993
AACGCAG103850.022.4146656
GGTATCA42000.022.259391
CGGGATA4450.022.25105917
AGTACGG63800.022.1169713
ACGGGAT13850.022.04459816
GTCCGGA752.8727567E-421.99961755
GAGTACG64800.021.77566112
GGCCTAT650.00324168121.1514571
TACGGGG26450.020.79856115
Produced by FastQC (version 0.11.5)