FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697333
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC64360.23860606013421407No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA54950.20371974835884185No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT50710.18800051754825972No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC47530.1762110944403231No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA43300.16052893728731304No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC42440.15734060273611009No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA41070.15226151164872856No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.15085271266098774No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.14836877760365516No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT36710.13609739694728087No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT36140.1339841984656696No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG34650.12846022348742256No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT33430.12393723726362299No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT32310.1197849876155447No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA31310.11607762185833191No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA29720.1101829103043636No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA29720.1101829103043636No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT28450.1054745557927034No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA28260.10477015629883296No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT28200.10454771435340018No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG27150.10065498030832679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC33350.041.925742
GTATCAA120850.039.8992271
GTGGTAT34800.039.83241
AACGCAG136000.035.4049266
TCAACGC140300.034.381034
ATCAACG143000.033.7913173
CAACGCA142500.033.7905925
ACGCAGA150350.032.0822687
CGCAGAG150850.031.9201778
TATCAAC159500.030.3755992
GGTATCA65050.030.0682831
GCAGAGT163550.029.417169
GTCGAGG13750.026.2722288
ATTAGAG24600.026.0869053
GGCACCG24550.025.1027539
GGTACGG3250.024.8456823
CAGAGTA109400.024.10667810-11
GAGGCAC26600.023.0066577
GCTTGTA13250.022.7845551
ACGGGTC8800.022.2154885
Produced by FastQC (version 0.11.5)