FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907014
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41790.46074261257268356No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.42270571347300046No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.27320416222902844No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.23108794351575612No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.17397746892550722No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.17055966060060815No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC12790.1410121563724485No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA12670.1396891337950682No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT11130.12271034405202125No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10620.11708749809815505No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.11157490402573719No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10030.11058263709270198No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.10484953925738742No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.10231374598407522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23150.047.1798022
GTGGTAT23800.044.303731
GTATCAA78450.039.5635721
TATCAAC88800.036.133332
ATCAACG97800.032.8081783
TCAACGC98050.032.6378364
AACGCAG97300.032.5836686
GACAGGT5100.032.513221
GCAGCGT1605.456968E-1231.8879661
CAACGCA100400.031.7469165
GGTATCA40400.030.7303181
CGTCAGA1751.8189894E-1129.1418555
CGCAGAG110450.028.6289338
ACGCAGA111050.028.587497
AAGTACG2100.028.344861
GCAGAGT114200.027.7648039
GTCGAGG15450.026.4083168
CAGAGTA56200.025.18429410-11
TCGAGGT15250.024.805169
AGTCGAG22200.023.929367
Produced by FastQC (version 0.11.5)