FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907014
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16540.18235661191558233No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11880.13097923516064802No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10800.11907203196422547No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC9840.10848785134518321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29200.037.7442441
TGGTATC29650.036.9717252
CGTGCTA507.022067E-427.50582511
GTATCAA94700.025.2208081
AGAGTAC57250.024.88736311
CAGAGTA57550.024.70983710
TTAAACG1451.4551915E-1124.66311622
CGGGTTA801.6051095E-524.06759617
CGGGGTA2350.023.40921217
TATCAAC103900.022.8993872
TTACGCA600.00204577722.8962443
GGTATCA50250.022.800563
ATCAACG112200.021.255553
AACGCAG111150.021.2338736
TCGGTAC650.003233182721.1606624
TACGCAG650.003256018321.1349954
AGTACGG66050.021.07183513
TCAACGC112600.021.0336384
GAGTACG67250.020.98213812
CAACGCA114700.020.7204045
Produced by FastQC (version 0.11.5)