FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187168
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80810.3694732183353085No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT73440.33577667559144975No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.21969048559598534No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.19093183514023612No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT32290.1476338351694977No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA32230.1473595078201583No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.14356464615429634No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA26630.12175562188181246No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC26010.11892090593863845No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT25320.11576614142123515No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG23980.10963949728598808No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC22120.10113534945646607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC61900.036.456512
GTATCAA184750.035.8133241
GTGGTAT64050.034.5890051
AACGCAG207350.032.6704756
TATCAAC213800.031.8438822
AAGTACG4850.030.6863881
TCAACGC224700.030.3180754
ATCAACG224700.030.2991623
CAACGCA227800.029.8868395
GCTTGTA13450.029.402221
ACGCAGA231850.029.2364587
CGCAGAG233900.028.9445388
GTCGAGG25800.027.0150188
GCAGAGT251450.026.9756729
GACAGGT8850.026.9069351
ACAGGTA10800.026.7734681
GGTATCA109950.025.9505621
CAGAGTA142850.023.26657310-11
CGGGGTC13900.022.0134894
ACAGGCC3200.021.2612821
Produced by FastQC (version 0.11.5)