FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599101
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.4611910178751162No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.45518201438488665No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16780.28008632935014294No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.25304581364411005No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.2034715348497165No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.1704220156534541No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.15189425489191305No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC7210.12034698656820803No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA7140.11917856922288561No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.10749439576966155No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.10749439576966155No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.10315455991560687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15850.049.067622
GTGGTAT15700.047.1198651
GTATCAA53300.039.8040921
TATCAAC62400.035.347272
GTCGAGG9150.034.3703358
AACGCAG67200.032.6959846
ATCAACG68150.032.552013
TCAACGC68150.032.552014
CAACGCA69350.031.9887455
AAGTACG2400.031.8871481
TCGAGGT10150.030.9840959
GGTATCA28150.030.3579271
ACGCAGA77400.028.4421227
ATACGGG905.1376974E-528.3441331
TTAGTCG300.006804131428.3346766-67
GTAGCGA600.00680422928.3323049
GCCACGC600.00680422928.3323048
CGCAGAG77950.028.1323978
GCAGAGT80000.027.4115059
CTATCGG1106.470278E-627.0557611
Produced by FastQC (version 0.11.5)