FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599101
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11650.1944580296143722No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT6570.10966431369668889No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6310.10532447784263421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21100.037.9081762
GTGGTAT21050.037.6127621
CGGGCTA300.001923939636.6703415
CGACGTC350.004101095731.42384743
CGGGATA801.499393E-830.93801517
GACAGGT2100.027.4912741
TATAACG400.0078823727.49127420
TCGGAGA507.0444465E-427.4889778
AGAGTAC40200.024.21884511
GTATCAA68500.024.1602131
CAGAGTA41800.023.68460710
GCTTACG600.002038154822.9093951
GGTATCA37250.022.7972283
TATCAAC75100.022.1796682
GCTGTAC752.8831055E-421.9856763
ACGGGTA1901.8189894E-1221.7108916
AACGCAG80550.020.7420486
AGTACGG46300.020.67161813
GAGTACG46900.020.6417312
ATCAACG81500.020.468263
Produced by FastQC (version 0.11.5)