FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756139
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59600.7882148652562558No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC350.00410568631.4177253
GTATCAA33650.028.2871951
AACCGTG606.307036E-527.50506254
GTGGTAT8000.026.8227621
TATCAAC37150.025.7583522
AACGCAG37500.025.5095066
AGAGTAC35450.024.9751811
TGGTATC8050.024.9358792
CAACGCA39000.024.6693385
TCAACGC38600.024.6417524
CAGAGTA36200.024.53044510
ATCAACG38850.024.4831813
CGCAGAG39450.024.3895538
ACGCAGA39600.024.2261457
ATACCGC600.002039761322.9072446
GGTATCA16300.022.6160141
GCAGAGT43050.022.4153469
CGACGGC752.8729643E-421.99822827
AGTACGG40600.021.26957313
GAGTACG41350.021.07913212
Produced by FastQC (version 0.11.5)