FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785063
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20770.26456475467573937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA150.0057971354.965924
GTGGTAT16700.044.2858851
GCTATAC257.8789657E-443.9727363
TGGTATC16550.043.839892
GTATCAA65350.027.0096511
AGAGTAC41550.025.35103411
GGGTAAT1001.3116005E-724.7441221
CAGAGTA43350.024.4252810
TATCAAC72350.024.1591762
GGTATCA33100.024.004871
GGAACGG600.00203399822.91852455
ACGGGGA7200.022.53655216
ATCAACG78000.022.373953
TCAACGC78300.022.2882254
CGGGTAT752.869958E-422.00178517
CAACGCA79750.021.9174465
GAGTACG48100.021.84168812
AGTACGG48000.021.82989713
AACGCAG82200.021.2307556
GACAGGT3950.020.1850781
Produced by FastQC (version 0.11.5)