FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371773
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.3562542782224173No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.31776394490925247No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.21461276756431277No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.17284200811650324No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.14171440901665217No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.12400010788957065No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA16090.11729345890318588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24350.050.791842
GTGGTAT24850.048.939691
GTATCAA88900.039.4136851
TATCAAC101250.035.1342242
GGTATCA47500.033.033481
ATCAACG107800.032.920593
TCAACGC107900.032.7719154
AACGCAG110150.032.178496
CAACGCA110950.031.9093275
GTCGAGG14300.030.9087458
TCGAGGT14550.028.9172049
CGCAGAG125050.028.3443478
ACGCAGA125150.028.2877397
GCAGAGT131500.026.9540739
CAGAGTA69350.023.77767210-11
AGTCGAG23100.023.7335017
AAGTACG2553.6379788E-1223.3458371
TACGGAG1102.0158468E-423.1824044
AGAGTAC65950.021.23365210-11
GAGTACG79350.020.3794212-13
Produced by FastQC (version 0.11.5)