FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371773
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23580.17189432945538366No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT14610.1065045018381321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29650.039.218442
GTGGTAT30100.039.2015271
GTATCAA108250.026.9841521
AGAGTAC72000.025.01829111
CAGAGTA73750.024.68566110
TATCAAC119500.024.384432
ATCAACG127800.022.6700633
TCAACGC128550.022.4308834
AACGCAG130500.022.2220926
GGTATCA54800.022.0204873
CAACGCA131250.022.0113355
AGTACGG82250.021.63302213
CGGGCTA1155.042675E-721.5225417
GAGTACG83250.021.50530412
CGGGTCG903.995205E-521.38968517
ACGGGCT3100.021.29111316
TACGGGG30450.019.9596915
ACGGGTC4850.019.84609816
TACGCGA1401.5294972E-719.64430229
ACGCAGA148550.019.4671257
Produced by FastQC (version 0.11.5)