FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966854
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.1907614901766984No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.18394857981324492No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.1229374422300791No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.10702370384380334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24950.039.542431
TGGTATC25700.038.6938132
GTATCAA76300.038.122161
TATCAAC86950.034.0660672
ATCAACG90500.032.4010663
TCAACGC90600.032.3183984
CAACGCA92900.031.5182655
AACGCAG93450.031.3782396
GGTATCA45100.030.8330961
ACGCAGA102650.028.5252917
CGCAGAG105500.027.6748438
GCAGAGT118550.024.6654959
GTCGAGG11500.022.9126198
CAGAGTA67800.022.75689510-11
AGAGTAC63300.021.25230810-11
AGTACGG80750.019.7354742
AGTCGAG18150.019.4344087
TACGGGT16650.019.3980544
GAGTACG82900.019.1850531
TACGGGG36450.017.9548572
Produced by FastQC (version 0.11.5)