FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662232
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA35000.5285156863455707No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.5245895698184322No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC26900.4062020560770244No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT24920.37630316867804636No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT24190.36527984150569587No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA23380.35304847847884124No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA21440.3237536090071153No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.3226965776344242No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC21120.31892146558909873No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG20970.31665639836190335No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA18950.2861534930356733No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA18480.2790562823904613No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG17450.2635028207637203No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC17120.2585196728638906No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT17050.25746264149119946No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG16770.2532345160004349No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT16770.2532345160004349No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG16310.24628830983703595No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC16240.2452312784643448No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA16220.24492926950071878No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT15940.2407011440099542No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.24009712608270214No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA15350.23179187958298603No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT14690.22182558378332667No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC14670.22152357481970064No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT14610.22061754792882254No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG14060.21231230142910643No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA13700.2068761400838377No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC13480.20355404148395123No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA13430.20279901907488615No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT12980.19600381739330025No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG12800.19328573672066585No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT12790.19313473223885286No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG12660.1911716739752836No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA12600.19026564708440546No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG11440.1727491271940951No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG11310.17078606893052586No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG11040.1667089479215743No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG10560.15946073279454936No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT10310.15568562074922385No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT9930.14994745044032906No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG9640.14556832046775148No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC9530.14390727116780827No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG9420.14224622186786504No Hit
CAGTAGCGGCGAGCGAACGGGGATCAGCCCATTAAGTTATATGTGTTTTA8850.13363896640452289No Hit
CCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAAC8650.13061887676826248No Hit
TTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTATCGCATGATGAA8620.13016586332282343No Hit
ACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTC8450.1275987871320021No Hit
GATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC8440.12744778265018905No Hit
GTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA8420.12714577368656302No Hit
ATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTC8400.12684376472293699No Hit
ATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCG8020.1211055944140422No Hit
CTATTACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCACATGCAC7970.1203505720049771No Hit
AGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG7870.11884052718684691No Hit
GGTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATG7590.11461240169608235No Hit
ATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATA7290.11008226724169173No Hit
ACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACA7190.10857222242356152No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT7130.10766619553268342No Hit
AAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGG7110.10736418656905737No Hit
CGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAG7110.10736418656905737No Hit
CTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATA7070.10676016864180529No Hit
GGTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCA7060.10660916415999226No Hit
CCCCGAAGCTTATCGCAGGCTATTACGTCTTTCATCGCCTCTGACTGCCA7060.10660916415999226No Hit
GTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCG6970.10525012382367509No Hit
GTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGAT6660.10056898488747146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12400.050.0440142
GTGGTAT12950.047.936691
GGTATCA29950.042.874111
GTATCAA56850.042.706171
TATCAAC64150.038.4946562
ATCAACG70400.035.313323
TCAACGC70450.035.288264
AACGCAG71200.034.8568576
CAACGCA71850.034.5415155
CAGTGGT1950.032.707071
TAGCCGG652.5533486E-432.689785
ACGCAGA77300.032.0512127
CGCAGAG77500.031.96858
GCAGAGT81050.030.5682749
ACAGACG703.9397497E-430.359382
AGCCGGT703.943238E-430.3547976
GACAGGT2250.028.3461271
CAGAGTA57150.027.58754310-11
GACAGAC2009.276846E-1125.5115131
GACAGGG1351.110142E-625.1965541
Produced by FastQC (version 0.11.5)