FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331200
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69740.5238882211538461No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.3938551682692307No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT34610.2599909855769231No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.25728665865384615No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.10990084134615385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16900.057.1004071
TGGTATC18150.053.159882
GTATCAA64650.040.768361
GGTATCA34050.037.079971
TATCAAC71800.036.4662442
ATCAACG76850.034.1239853
TCAACGC77300.033.8153654
CAACGCA78600.033.256085
AACGCAG80300.032.710826
ACGCAGA88750.029.5930447
CGCAGAG91250.028.7357048
GCAGAGT96000.027.4466929
CAGAGTA52650.026.59712810-11
AGAGTAC52050.022.33131410-11
GAGTACG63700.022.01711312-13
GTACGGG65500.021.34717814-15
ATTACTC2301.306762E-820.3274733
AGTACGG59000.019.629112-13
CCGGTCG1601.1734354E-418.592929
TACGGGG27450.018.34686514-15
Produced by FastQC (version 0.11.5)