FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331200
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71040.5336538461538461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9850.038.543431
TGGTATC11000.034.4957352
GTATCAA52900.030.3193861
GGTATCA23150.029.2342281
CCGTTAA400.00787254927.50073216
TATCAAC60200.027.1311972
AGAGTAC58800.025.4877111
ATCAACG64250.025.3315813
CAGAGTA59550.025.16670610
TCAACGC64850.024.9690914
CAACGCA64750.024.9651955
AACGCAG65300.024.924266
ACGCAGA68450.023.777277
CGCAGAG68200.023.622568
AGTACGG64050.022.88507513
GCAGAGT72950.022.2016539
GAGTACG66900.021.99153712
TACGGGG28250.021.12445415
GGACGTC804.4431104E-420.62477519
GTACGGG71850.020.20930714
Produced by FastQC (version 0.11.5)