FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985031
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99510.5013019947799304No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT93310.47006822563476336No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA81070.4084067200965627No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT73390.3697171479941623No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC72440.36493132852837057No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG65530.33012078904561193No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.3174761502465201No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA58320.29379893815260316No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT57790.29112895466116145No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA57380.2890634957338198No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC53570.26986984082364457No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG50510.2544544644390944No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA49240.248056579469036No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA49030.246998661481861No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA48750.24558810416562765No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.23893833396052758No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT46490.23420289154174417No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG46490.23420289154174417No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT42770.215462630054644No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT42360.21339717112730228No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT41730.21022341716577728No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG41630.20971964669569393No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT41580.20946776146065224No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC41520.20916549917860222No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC39910.2010547946102605No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC39840.20070215528120217No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA38520.1940523850761021No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.19138240158466038No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA36740.18508527070861866No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA35740.18004756600778526No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC35520.17893927097360193No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA34470.17364968103772688No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.17349854989670185No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG34260.17259176305055188No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG33960.17108045164030183No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG33510.1688134845249268No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT32400.16322163230700176No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT29270.14745361659339323No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG28190.14201289551649318No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG27810.1400985677301765No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG26260.13229012544388477No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG26150.1317359779267931No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG25530.12861260101227637No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG24920.12553960114476803No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT24500.123423765170418No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.12322225698238466No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC24380.12281924060631799No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT24290.12236584718324298No Hit
CCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAAC22900.11536343764908458No Hit
CAGTAGCGGCGAGCGAACGGGGATCAGCCCATTAAGTTATATGTGTTTTA22600.11385212623883456No Hit
GATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC22570.11370099509780955No Hit
ATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTC22260.1121393066405512No Hit
TTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTATCGCATGATGAA22170.1116859132174762No Hit
ACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTC21580.1087136674439845No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.10674896261065947No Hit
ATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCG20970.10564066757647614No Hit
ATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATA20930.1054391593884428No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.10528802824741779No Hit
GTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA20760.10458274958930111No Hit
CTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATA19930.10040145468760943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC52150.056.4744342
GTGGTAT51850.055.916491
GACAGGT6150.040.7831881
GTATCAA195950.039.2245031
TATCAAC220100.035.5282
GGTATCA103300.034.8567851
GTCGAGG28850.032.998728
TCAACGC239950.032.624354
ATCAACG240800.032.4738923
CGTCAGA2750.032.4533545
AACGCAG241350.032.4174966
CAACGCA247450.031.6527065
TCGAGGT29350.031.2796869
ACGCAGA272100.028.7383777
CGCAGAG273650.028.5459758
GCAGAGT286350.027.3258299
CAGTGGT9100.026.1607911
CAGAGTA154850.025.75964210-11
ATACGGG2600.024.5257431
GTACTGG6800.023.7563081
Produced by FastQC (version 0.11.5)