FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528024
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.351696046658953No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45040.29475976817118055No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.21072967440301985No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.16832196352936865No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.14338780019162004No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.10889881310764754No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.1015036413040633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23000.054.3236242
GTGGTAT23500.052.6493871
GTATCAA84050.038.748891
TATCAAC94200.034.4676482
GGTATCA45650.034.367961
TCAACGC96750.033.4713444
ATCAACG97550.033.283983
CAACGCA99350.032.5953985
AACGCAG103400.031.3186936
GTCGAGG17850.029.5224678
AGCGTCA2200.028.9759563
TCGAGGT18050.027.3117779
ACGCAGA119500.027.0991887
CGCAGAG121250.026.7080658
GCAGAGT124150.026.1868889
AGTCGAG24350.025.6559187
GCGTCAG2500.025.4988424
CAGAGTA61800.024.58504510-11
GACAGGT9600.024.359011
CGTCAGA2950.023.0497995
Produced by FastQC (version 0.11.5)