FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554919
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA22880.1471459285017419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22360.14380170285397503No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT15990.10283493866883099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC37850.032.2499732
GTGGTAT38700.031.6269251
GTATCAA126950.025.998991
GACAGGT4450.025.9595531
GCTTGTC2500.025.3043821
GCACCGC4100.023.47514210
AGAGTAC98500.023.39547311
CAGAGTA100400.023.0896810
TATCAAC145450.022.6630172
AACGCAG146000.022.4979786
TCAACGC152750.021.3778324
ATCAACG154050.021.322333
CAACGCA155950.020.9730745
GGCCGCG4700.020.48227140
GCTTGTA6850.020.4781
ACGCAGA162000.020.2935757
GGCACCG5050.020.1481139
CGCAGAG164000.019.997748
AACGGCC5100.019.95443737
CTGCTTA3650.019.58865218
Produced by FastQC (version 0.11.5)