FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582600
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.2689245545305194No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.25407557184380136No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.17641855175028434No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.1500695058764059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22800.053.4949042
GTGGTAT24900.049.349581
GTATCAA86900.038.947381
GGTATCA47550.034.873781
TATCAAC99050.034.3671382
ATCAACG104800.032.3598863
TCAACGC104950.032.3136374
AACGCAG107300.031.6455356
CAACGCA107400.031.57655
ACGCAGA118850.028.5344227
CGCAGAG120150.028.2964258
GCAGAGT128050.026.5838769
GTCGAGG13250.024.6967748
CAGAGTA75700.023.41133110-11
GCGTTAC1102.0102269E-423.1922821
TATACCG1102.0171251E-423.180565
AGAGTAC73250.020.45207810-11
CCGCGTC1050.00418865620.2369989
TCGAGGT16600.019.4567769
AGTCGAG23300.018.9689277
Produced by FastQC (version 0.11.5)